Hotspot

rps16

LSC · 4498-5606 · 1109 bp

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Pi 0.0123
MarkerSeek score 48.90
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1109
variable_sites
27
pi
0.0123
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 AGGAATACTAGTGATCTTCTCCT ACCACTTTGCAATGAAAAGAGG 39.1 40.9 55.4 57.7 1168-1191 bp 1.0 43.5
rps16_p2 AGGAATACTAGTGATCTTCTCCT CCACTTTGCAATGAAAAGAGGA 39.1 40.9 55.4 57.4 1167-1190 bp 1.0 43.5
rps16_p3 AGGAATACTAGTGATCTTCTCCTACT ACCACTTTGCAATGAAAAGAGG 38.5 40.9 57.9 57.7 1168-1191 bp 1.0 43.5
rps16_p4 AGGAATACTAGTGATCTTCTCC ACCACTTTGCAATGAAAAGAGG 40.9 40.9 53.9 57.7 1168-1191 bp 1.0 43.5
rps16_p5 GGAATACTAGTGATCTTCTCCT ACCACTTTGCAATGAAAAGAGG 40.9 40.9 53.9 57.7 1167-1190 bp 1.0 43.5