Hotspot

ndhA

SSC · 111402-113540 · 2139 bp

Pi 0.00902

MarkerSeek score 56.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2139
variable_sites: 42
pi: 0.00902
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	TATGGGGGAAGTTGACCGTT	47.6	50.0	58.1	58.6	2274-2290 bp	1.0	68.4
ndhA_p2	ACCCAGTTACCATAGGGAACA	TTATGGGGGAAGTTGACCGT	47.6	50.0	58.1	58.6	2275-2291 bp	1.0	68.4
ndhA_p3	ACCCAGTTACCATAGGGAACA	ATTATGGGGGAAGTTGACCG	47.6	50.0	58.1	57.3	2276-2292 bp	1.0	67.7
ndhA_p4	ACCCAGTTACCATAGGGAACA	TATGGGGGAAGTTGACCGT	47.6	52.6	58.1	57.9	2274-2290 bp	1.0	64.8
ndhA_p5	CCCAGTTACCATAGGGAACA	TATGGGGGAAGTTGACCGTT	50.0	50.0	56.2	58.6	2273-2289 bp	1.0	62.8