Hotspot

rpl32-trnL-UAG

SSC · 105119-105867 · 749 bp

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Pi 0.0206
MarkerSeek score 63.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
749
variable_sites
37
pi
0.0206
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGGTTTTTCTGGGCAACA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.1 58.0 765-866 bp 1.0 86.5
rpl32-trnL-UAG_p2 AGGGTTTTTCTGGGCAACAA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.1 58.0 764-865 bp 1.0 86.5
rpl32-trnL-UAG_p3 AAGGGTTTTTCTGGGCAACA GGATTCGAACCGAGATGCTT 45.0 50.0 58.1 58.1 768-869 bp 1.0 86.0
rpl32-trnL-UAG_p4 AGGGTTTTTCTGGGCAACAA GGATTCGAACCGAGATGCTT 45.0 50.0 58.1 58.1 767-868 bp 1.0 86.0
rpl32-trnL-UAG_p5 AGGGTTTTTCTGGGCAACAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 737-838 bp 1.0 85.6