Hotspot

ndhF-rpl32

SSC · 104057-104938 · 882 bp

Pi 0.0158

MarkerSeek score 59.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 882
variable_sites: 32
pi: 0.0158
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GAAGAGGGAATTGTGCGGAT	GGTTTTGGATGCTCATTGCC	50.0	50.0	58.0	58.0	1759-1786 bp	1.0	82.1
ndhF-rpl32_p2	CATTCCCACGAGTTCCCAAA	GGTTTTGGATGCTCATTGCC	50.0	50.0	58.1	58.0	1488-1515 bp	1.0	82.0
ndhF-rpl32_p3	AACACATTCCCACGAGTTCC	GGTTTTGGATGCTCATTGCC	50.0	50.0	58.1	58.0	1492-1519 bp	1.0	81.9
ndhF-rpl32_p4	GAAGAGGGAATTGTGCGGAT	ATGCTCATTGCCACTGGAAA	50.0	45.0	58.0	58.1	1751-1778 bp	1.0	81.6
ndhF-rpl32_p5	GAAGAGGGAATTGTGCGGAT	TTTGGATGCTCATTGCCACT	50.0	45.0	58.0	58.1	1756-1783 bp	1.0	81.6