Hotspot

rpl22-rps19

IRb · 80137-80205 · 69 bp

Pi 0.0492

MarkerSeek score 63.90

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 69
variable_sites: 6
pi: 0.0492
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TCTTCTTCTTTATCCTAGGGA	CAAATTGGGGGAATTCGTGC	38.1	50.0	52.4	58.0	193-206 bp	1.0	44.0
rpl22-rps19_p2	TCTTCTTCTTTATCCTAGGGA	GCAAATTGGGGGAATTCGTG	38.1	50.0	52.4	58.0	194-207 bp	1.0	44.0
rpl22-rps19_p3	TTCTTCTTCTTTATCCTAGGGA	CAAATTGGGGGAATTCGTGC	36.4	50.0	53.3	58.0	194-207 bp	1.0	44.0
rpl22-rps19_p4	TTCTTCTTCTTTATCCTAGGGA	GCAAATTGGGGGAATTCGTG	36.4	50.0	53.3	58.0	195-208 bp	1.0	43.9
rpl22-rps19_p5	TTTCTTCTTCTTTATCCTAGGGA	CAAATTGGGGGAATTCGTGC	34.8	50.0	54.2	58.0	195-208 bp	1.0	43.9