Hotspot

rps8-rpl14

LSC · 76769-76913 · 145 bp

Pi 0.0129

MarkerSeek score 62.90

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 145
variable_sites: 5
pi: 0.0129
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TCAGCAATAGTGTCCTTGCC	CGATTGCCGAGGAATTGAGA	50.0	50.0	57.9	58.1	229-271 bp	1.0	82.6
rps8-rpl14_p2	GCAATAGTGTCCTTGCCCAT	CGATTGCCGAGGAATTGAGA	50.0	50.0	58.2	58.1	226-268 bp	1.0	81.7
rps8-rpl14_p3	TCAGCAATAGTGTCCTTGCC	AGGAACTCGAGTTTTTGGCG	50.0	50.0	57.9	58.8	249-291 bp	1.0	76.6
rps8-rpl14_p4	CAGCAATAGTGTCCTTGCCC	CGATTGCCGAGGAATTGAGA	55.0	50.0	58.9	58.1	228-270 bp	1.0	76.3
rps8-rpl14_p5	GCAATAGTGTCCTTGCCCAT	AGGAACTCGAGTTTTTGGCG	50.0	50.0	58.2	58.8	246-288 bp	1.0	75.7