Hotspot

psbE-petL

LSC · 62083-63358 · 1276 bp

Pi 0.0155

MarkerSeek score 69.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1276
variable_sites: 45
pi: 0.0155
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GCCAATAAATAGAGCTGGGGT	45.0	47.6	58.0	57.8	1320-1482 bp	1.0	76.5
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCCAATAAATAGAGCTGGGGT	50.0	47.6	57.9	57.8	1309-1471 bp	1.0	76.2
psbE-petL_p3	ACCAACCCGCAATGAATAGG	GCCAATAAATAGAGCTGGGGT	50.0	47.6	58.2	57.8	1311-1473 bp	1.0	74.8
psbE-petL_p4	TGACAAATAACCAACCCGCA	AGCCAATAAATAGAGCTGGGG	45.0	47.6	58.0	57.5	1321-1483 bp	1.0	74.2
psbE-petL_p5	CAACCCGCAATGAATAGGGA	AGCCAATAAATAGAGCTGGGG	50.0	47.6	57.9	57.5	1310-1472 bp	1.0	74.0