Hotspot

petA-psbJ

LSC · 60468-61313 · 846 bp

Pi 0.0165

MarkerSeek score 65.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 34
pi: 0.0165
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	ACTGTAGCTGGTATTGCTGTG	50.0	47.6	57.9	58.0	931-1030 bp	1.0	78.1
petA-psbJ_p2	TGTTCTTCTTCGCATCCGTT	ACTGTAGCTGGTATTGCTGTG	45.0	47.6	57.8	58.0	924-1023 bp	1.0	77.2
petA-psbJ_p3	TTGTTCTTCTTCGCATCCGT	ACTGTAGCTGGTATTGCTGTG	45.0	47.6	57.8	58.0	925-1024 bp	1.0	77.2
petA-psbJ_p4	GGCCTTTTGTTCTTCTTCGC	CTGTAGCTGGTATTGCTGTGA	50.0	47.6	57.9	57.7	930-1029 bp	1.0	76.3
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	TGGCTGATAGGTACTGTAGCT	50.0	47.6	57.9	57.7	943-1042 bp	1.0	75.7