Hotspot

trnT-UGU-trnL-UAA

LSC · 45432-46263 · 832 bp

Pi 0.0119

MarkerSeek score 55.10

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 832
variable_sites: 21
pi: 0.0119
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	TAAAACCTGGCCTGTTGACC	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.0	58.0	1498-1525 bp	1.0	82.9
trnT-UGU-trnL-UAA_p2	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.0	58.0	922-961 bp	1.0	82.8
trnT-UGU-trnL-UAA_p3	TAAAACCTGGCCTGTTGACC	TAGTTCGAGAACCCCCTTGT	50.0	50.0	58.0	58.0	1621-1648 bp	1.0	82.6
trnT-UGU-trnL-UAA_p4	TAAAACCTGGCCTGTTGACC	GTTCGAGAACCCCCTTGTTT	50.0	50.0	58.0	58.0	1619-1646 bp	1.0	82.5
trnT-UGU-trnL-UAA_p5	ATTTGAACCGATGACCCTCG	TAGTTCGAGAACCCCCTTGT	50.0	50.0	58.0	58.0	1045-1084 bp	1.0	82.4