Hotspot

ycf3-trnS-GGA

LSC · 43482-44097 · 616 bp

Pi 0.012

MarkerSeek score 63.00

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 616
variable_sites: 16
pi: 0.012
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TCTCCTGAGGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.1	58.2	501-807 bp	1.0	84.0
ycf3-trnS-GGA_p2	TCTCCTGAGGTTGTCGGAAT	TTCCAATGCTACGCCTTCAA	50.0	45.0	58.1	57.8	451-757 bp	1.0	83.8
ycf3-trnS-GGA_p3	TCTCCTGAGGTTGTCGGAAT	AGTTCCAATGCTACGCCTTC	50.0	50.0	58.1	58.3	453-759 bp	1.0	83.2
ycf3-trnS-GGA_p4	TTCTCCTGAGGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	502-808 bp	1.0	82.2
ycf3-trnS-GGA_p5	TTTCTCCTGAGGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	503-809 bp	1.0	82.2