Hotspot

trnC-GCA-rpoB

LSC · 18205-19381 · 1177 bp

Pi 0.0164

MarkerSeek score 60.10

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1177
variable_sites: 47
pi: 0.0164
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	ATTCCCTCGTTTCCATTCCG	50.0	50.0	58.1	58.0	1235-1269 bp	1.0	84.1
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TGTGGACATTCCCTCGTTTC	50.0	50.0	58.1	57.8	1242-1276 bp	1.0	82.9
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	TGTCAAGTTCTTCTGCCAGG	50.0	50.0	58.1	57.7	1318-1352 bp	1.0	82.0
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1250-1284 bp	1.0	81.7
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1249-1283 bp	1.0	81.7