Hotspot

trnG-GCC-trnfM-CAU

LSC · 12221-12668 · 448 bp

Pi 0.0209

MarkerSeek score 58.80

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 448
variable_sites: 20
pi: 0.0209
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGGAGAAGATACGGGTTCGA	CGAGGCTCATAACCTTGAGG	50.0	55.0	57.6	57.8	373-538 bp	1.0	81.8
trnG-GCC-trnfM-CAU_p2	AGGAGAAGATACGGGTTCGA	CAGTTTGGTAGCTCACGAGG	50.0	55.0	57.6	58.3	388-553 bp	1.0	81.3
trnG-GCC-trnfM-CAU_p3	AGGAGAAGATACGGGTTCGA	GAGCGGAGTAGAGCAGTTTG	50.0	55.0	57.6	58.4	401-566 bp	1.0	80.6
trnG-GCC-trnfM-CAU_p4	AGGAGAAGATACGGGTTCGA	AGAGCGGAGTAGAGCAGTTT	50.0	50.0	57.6	58.4	402-567 bp	1.0	79.9
trnG-GCC-trnfM-CAU_p5	TTGCCAAGGAGAAGATACGG	CGAGGCTCATAACCTTGAGG	50.0	55.0	57.3	57.8	379-544 bp	1.0	79.7