Hotspot

psbZ-trnG-GCC

LSC · 11863-12149 · 287 bp

Pi 0.0319

MarkerSeek score 57.00

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 287
variable_sites: 21
pi: 0.0319
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	AGTCTTTCTCGTAGCTATTCTGA	TACCATTGAACTACGCTCGC	39.1	50.0	56.6	58.1	342-359 bp	1.0	52.2
psbZ-trnG-GCC_p2	AGTCTTTCTCGTAGCTATTCTGAA	TACCATTGAACTACGCTCGC	37.5	50.0	57.2	58.1	342-359 bp	1.0	49.4
psbZ-trnG-GCC_p3	GTCTTTCTCGTAGCTATTCTGA	TACCATTGAACTACGCTCGC	40.9	50.0	55.2	58.1	341-358 bp	1.0	48.8
psbZ-trnG-GCC_p4	AGTCTTTCTCGTAGCTATTCTG	TACCATTGAACTACGCTCGC	40.9	50.0	55.1	58.1	342-359 bp	1.0	48.7
psbZ-trnG-GCC_p5	TCGTAGCTATTCTGAATTCTCTCA	TACCATTGAACTACGCTCGC	37.5	50.0	57.1	58.1	334-351 bp	1.0	48.4