Hotspot

trnS-GCU-psbD

LSC · 7695-8661 · 967 bp

Pi 0.0065

MarkerSeek score 54.60

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 967
variable_sites: 16
pi: 0.0065
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1106-1139 bp	1.0	80.2
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1093-1126 bp	1.0	79.6
trnS-GCU-psbD_p3	ATTAGCAATCCGCCGCTTTA	CCCTTCGTAACCAGTCATCC	45.0	55.0	58.0	57.7	1077-1110 bp	1.0	79.1
trnS-GCU-psbD_p4	GAGCGGAAAGAGAGGGATTC	GCCGGACCATCCTACAAAAA	55.0	50.0	57.8	58.2	1157-1190 bp	1.0	78.5
trnS-GCU-psbD_p5	GAGCGGAAAGAGAGGGATTC	ACAAAAACGAAACGGTCCCT	55.0	45.0	57.8	58.2	1144-1177 bp	1.0	78.0