Pi
0.0051
MarkerSeek score
42.60
Primer available
yes
Label
ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf1_p1 | TCGAATTGAGAGATTAATCGGACA | TCTGACTGATAGAACAAGCACA | 37.5 | 40.9 | 57.9 | 57.1 | 189 bp | 1.0 | 42.1 |
| ycf1_p2 | TCGAATTGAGAGATTAATCGGACA | GACGGATCTGACTGATAGAACA | 37.5 | 45.5 | 57.9 | 57.1 | 195 bp | 1.0 | 42.1 |
| ycf1_p3 | TCGAATTGAGAGATTAATCGGACA | GGATCTGACTGATAGAACAAGCA | 37.5 | 43.5 | 57.9 | 58.0 | 192 bp | 1.0 | 42.1 |
| ycf1_p4 | TCGAATTGAGAGATTAATCGGACA | ACGGATCTGACTGATAGAACA | 37.5 | 42.9 | 57.9 | 55.9 | 194 bp | 1.0 | 42.1 |
| ycf1_p5 | TCGAATTGAGAGATTAATCGGACA | AGACGGATCTGACTGATAGAAC | 37.5 | 45.5 | 57.9 | 56.8 | 196 bp | 1.0 | 42.0 |