Hotspot

ndhF

SSC · 110929-113157 · 2229 bp

Back to Plumeria

Pi 0.00359

MarkerSeek score 48.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 12
pi: 0.00359
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	TTCAAACTCGGGAAAAGGGG	50.0	50.0	58.0	58.0	2767-2813 bp	1.0	81.5
ndhF_p2	GCAGAATACCGTCACCCATT	TTCAAACTCGGGAAAAGGGG	50.0	50.0	58.0	58.0	2891-2937 bp	1.0	81.4
ndhF_p3	AAGAGGGATCCACCGAAGAA	TTCAAACTCGGGAAAAGGGG	50.0	50.0	58.0	58.0	2759-2805 bp	1.0	81.4
ndhF_p4	AGGGTGGAAAGTGAGGAAGA	TTCAAACTCGGGAAAAGGGG	50.0	50.0	57.9	58.0	2832-2878 bp	1.0	80.8
ndhF_p5	AGGGATCCACCGAAGAAGAT	TTCAAACTCGGGAAAAGGGG	50.0	50.0	57.8	58.0	2756-2802 bp	1.0	80.4