Hotspot

clpP

LSC · 71225-73255 · 2031 bp

Back to Plumeria

Pi 0.00394

MarkerSeek score 47.30

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2031
variable_sites: 12
pi: 0.00394
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ACATGTTCCTCGACGTTGAG	TTCGTTACAGTCCCTCCTGT	50.0	50.0	57.9	58.0	2971-2974 bp	1.0	79.8
clpP_p2	TGCACCAACGGAAACCATAA	ATACCAAGGCAAACCCATGG	45.0	50.0	58.0	58.1	2923-2925 bp	1.0	79.2
clpP_p3	TTGCACCAACGGAAACCATA	ATACCAAGGCAAACCCATGG	45.0	50.0	58.0	58.1	2924-2926 bp	1.0	79.2
clpP_p4	ACATGTTCCTCGACGTTGAG	ATACCAAGGCAAACCCATGG	50.0	50.0	57.9	58.1	2729-2732 bp	1.0	78.8
clpP_p5	ACATGTTCCTCGACGTTGAG	GTCCCTCCTGTGATACTCCA	50.0	55.0	57.9	57.8	2962-2965 bp	1.0	78.6