Hotspot

ndhD-psaC

SSC · 117930-118044 · 115 bp

Back to Plumeria

Pi 0.0754

MarkerSeek score 68.50

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 115
variable_sites: 13
pi: 0.0754
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AACCCGCAGATATTGGCAAA	TGTGAATCTGCCTGTCCAAC	45.0	50.0	58.2	58.1	257 bp	1.0	82.0
ndhD-psaC_p2	ACCCGCAGATATTGGCAAAA	TGTGAATCTGCCTGTCCAAC	45.0	50.0	58.2	58.1	256 bp	1.0	82.0
ndhD-psaC_p3	CCCGCAGATATTGGCAAAAC	TGTGAATCTGCCTGTCCAAC	50.0	50.0	57.8	58.1	255 bp	1.0	81.6
ndhD-psaC_p4	ACCCGCAGATATTGGCAAAA	GGAAGACTGCGTTGGTTGTA	45.0	50.0	58.2	58.1	281 bp	1.0	81.5
ndhD-psaC_p5	AACCCGCAGATATTGGCAAA	GGAAGACTGCGTTGGTTGTA	45.0	50.0	58.2	58.1	282 bp	1.0	81.4