Hotspot

rpl2-rpl23

IRb · 87001-87018 · 18 bp

Back to Plumeria

Pi 0.037

MarkerSeek score 63.60

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: IRb
length_bp: 18
variable_sites: 1
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	CCACAACGATGCTGTCCATA	CGCTTCAACCGGGTTATTCT	50.0	50.0	58.0	58.3	181 bp	1.0	78.1
rpl2-rpl23_p2	ACTGTCTACGGTTCCATTGC	CGCTTCAACCGGGTTATTCT	50.0	50.0	57.9	58.3	128 bp	1.0	77.7
rpl2-rpl23_p3	CCACAACGATGCTGTCCATA	ATCATTACGCTTCAACCGGG	50.0	50.0	58.0	58.3	188 bp	1.0	77.6
rpl2-rpl23_p4	CCACAACGATGCTGTCCATA	TCCGGGAAAGGGTAGAAGAA	50.0	50.0	58.0	57.7	249 bp	1.0	77.5
rpl2-rpl23_p5	CCACAACGATGCTGTCCATA	TTCCGGGAAAGGGTAGAAGA	50.0	50.0	58.0	57.7	250 bp	1.0	77.5