Hotspot

trnP-UGG-psaJ

LSC · 67924-68274 · 351 bp

Back to Plumeria

Pi 0.0076
MarkerSeek score 49.90
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
351
variable_sites
4
pi
0.0076
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC GTACTTAGTACTGGCGCCAC 50.0 55.0 58.1 58.1 453 bp 1.0 79.3
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA GTACTTAGTACTGGCGCCAC 50.0 55.0 58.1 58.1 452 bp 1.0 79.2
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC GTACTTAGTACTGGCGCCAC 50.0 55.0 58.1 58.1 464 bp 1.0 79.2
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA GTACTTAGTACTGGCGCCAC 50.0 55.0 58.1 58.1 463 bp 1.0 79.2
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG GTACTTAGTACTGGCGCCAC 50.0 55.0 58.1 58.1 466 bp 1.0 79.2