Hotspot

psbE-petL

LSC · 66038-67127 · 1090 bp

Back to Plumeria

Pi 0.00917

MarkerSeek score 51.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1090
variable_sites: 15
pi: 0.00917
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AAAGGATCAAAACGGCCAGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1880-1881 bp	1.0	80.6
psbE-petL_p2	AAGGATCAAAACGGCCAGTT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1879-1880 bp	1.0	80.6
psbE-petL_p3	AAAGGATCAAAACGGCCAGT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	1858-1859 bp	1.0	80.5
psbE-petL_p4	AAGGATCAAAACGGCCAGTT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	1857-1858 bp	1.0	80.5
psbE-petL_p5	TCGTTCGGGTTTGATTGTGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	2089-2090 bp	1.0	80.2