Hotspot

ycf4-cemA

LSC · 61706-62398 · 693 bp

Back to Plumeria

Pi 0.00292

MarkerSeek score 62.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 693
variable_sites: 3
pi: 0.00292
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	GAGAGAGAAAGCCACCAAGG	45.0	55.0	58.1	57.9	792-800 bp	1.0	79.8
ycf4-cemA_p2	AAAAAGCTGCTGAATTGGCC	GAGAGAGAAAGCCACCAAGG	45.0	55.0	57.8	57.9	806-814 bp	1.0	79.1
ycf4-cemA_p3	AAAGCTGCTGAATTGGCCTA	GAGAGAGAAAGCCACCAAGG	45.0	55.0	57.8	57.9	804-812 bp	1.0	78.8
ycf4-cemA_p4	AAGCTGCTGAATTGGCCTAT	GAGAGAGAAAGCCACCAAGG	45.0	55.0	57.6	57.9	803-811 bp	1.0	76.9
ycf4-cemA_p5	AGCTGCTGAATTGGCCTATT	GAGAGAGAAAGCCACCAAGG	45.0	55.0	57.6	57.9	802-810 bp	1.0	76.9