Hotspot

atpH-atpI

LSC · 13237-14394 · 1158 bp

Back to Plumeria

Pi 0.00432

MarkerSeek score 64.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1158
variable_sites: 7
pi: 0.00432
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1219-1247 bp	1.0	77.7
atpH-atpI_p2	TACCCTCTACAGCTTGACCC	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.1	58.5	1302-1330 bp	1.0	76.8
atpH-atpI_p3	GCGATACCCTCTACAGCTTG	TTTTTGCAACTTTAGCCGCG	55.0	45.0	57.9	58.5	1306-1334 bp	1.0	76.8
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	1220-1248 bp	1.0	76.6
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	CCGCGGCTTATATAGGTGAA	45.0	50.0	58.0	57.3	1204-1232 bp	1.0	76.0