Hotspot

ycf2

IRa · 159189-166169 · 6981 bp

Back to Plumbago

Pi 0.0341

MarkerSeek score 63.20

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRa
length_bp: 6981
variable_sites: 348
pi: 0.0341
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	ATCGATGGGCGTGAAAAGAA	GAAATGCGTTGAGAAAGGGC	45.0	50.0	57.9	58.0	805-808 bp	0.666667	70.1
ycf2_p2	ATGAAATCGATGGGCGTGAA	GAAATGCGTTGAGAAAGGGC	45.0	50.0	58.0	58.0	810 bp	0.333333	52.9
ycf2_p3	CGCATCCGTAGTCTCATCAG	GAAATGCGTTGAGAAAGGGC	55.0	50.0	58.0	58.0	1483 bp	0.333333	52.8
ycf2_p4	ATCGATGGGATCTGGTAGGG	GAAATGCGTTGAGAAAGGGC	55.0	50.0	58.1	58.0	847 bp	0.333333	52.7
ycf2_p5	AATCGATGGGCGTGAAAAGA	GAAATGCGTTGAGAAAGGGC	45.0	50.0	57.9	58.0	806 bp	0.333333	52.3