Hotspot

trnI-GAU-rrn16

IRa · 150289-150580 · 292 bp

Back to Plumbago

Pi 0.0616

MarkerSeek score 81.30

Primer available yes

Label trnI-GAU-rrn16

Feature Metadata

label_name: trnI-GAU-rrn16
feature_type: igs
region: IRa
length_bp: 292
variable_sites: 27
pi: 0.0616
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU-rrn16_p1	CGGATGGGCACATTGAACTA	CAAGGTAGCCGTACTGGAAG	50.0	55.0	58.0	57.7	434-435 bp	1.0	84.5
trnI-GAU-rrn16_p2	CGGATGGGCACATTGAACTA	AGTGACTGGAGTGAAGTCGT	50.0	50.0	58.0	58.3	456-457 bp	1.0	84.1
trnI-GAU-rrn16_p3	CGGATGGGCACATTGAACTA	ACAAGGTAGCCGTACTGGAA	50.0	50.0	58.0	58.4	435-436 bp	1.0	84.0
trnI-GAU-rrn16_p4	CGGATGGGCACATTGAACTA	AAGTCGTAACAAGGTAGCCG	50.0	50.0	58.0	57.6	443-444 bp	1.0	83.9
trnI-GAU-rrn16_p5	CGGATGGGCACATTGAACTA	AACAAGGTAGCCGTACTGGA	50.0	50.0	58.0	58.4	436-437 bp	1.0	83.9