Hotspot

psbN-psbH

LSC · 82027-82321 · 295 bp

Back to Plumbago

Pi 0.129

MarkerSeek score 78.60

Primer available yes

Label psbN-psbH

Feature Metadata

label_name: psbN-psbH
feature_type: igs
region: LSC
length_bp: 295
variable_sites: 56
pi: 0.129
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbH_p1	GATGGTGACTAGGGTTGCTG	CACTCTCGACGGTTTGTGTA	55.0	50.0	58.0	57.6	347-458 bp	1.0	86.8
psbN-psbH_p2	AGATGGTGACTAGGGTTGCT	CACTCTCGACGGTTTGTGTA	50.0	50.0	58.0	57.6	348-459 bp	1.0	86.7
psbN-psbH_p3	GGTGACTAGGGTTGCTGTTT	CACTCTCGACGGTTTGTGTA	50.0	50.0	57.7	57.6	344-455 bp	1.0	84.8
psbN-psbH_p4	GATGGTGACTAGGGTTGCTG	CTCTCGACGGTTTGTGTAGC	55.0	55.0	58.0	58.7	345-456 bp	1.0	84.8
psbN-psbH_p5	AGATGGTGACTAGGGTTGCT	CTCTCGACGGTTTGTGTAGC	50.0	55.0	58.0	58.7	346-457 bp	1.0	84.7