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rps12-clpP

LSC · 75717-77272 · 1556 bp

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Pi 0.286

MarkerSeek score 80.30

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 1556
variable_sites: 549
pi: 0.286
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CTGAACGAGTCGCACATACA	ATGCCTTTCTGTCAGCAACA	50.0	45.0	58.0	58.0	1463-1744 bp	1.0	91.7
rps12-clpP_p2	ATCGAGCTGATTGGGTTTCC	ATGCCTTTCTGTCAGCAACA	50.0	45.0	58.0	58.0	1628-1909 bp	1.0	91.5
rps12-clpP_p3	CTGAACGAGTCGCACATACA	AAGCAACGGAGCCATCATAG	50.0	50.0	58.0	58.0	1888-2168 bp	1.0	91.5
rps12-clpP_p4	ATCGAGCTGATTGGGTTTCC	AAGCAACGGAGCCATCATAG	50.0	50.0	58.0	58.0	2053-2333 bp	1.0	91.3
rps12-clpP_p5	TAGTGACCGGTTCTTCGAGT	ATGCCTTTCTGTCAGCAACA	50.0	45.0	58.1	58.0	1549-1830 bp	1.0	91.1