Hotspot

trnS-GCU-trnG-UCC

LSC · 9334-10383 · 1050 bp

Back to Plumbago

Pi 0.0449

MarkerSeek score 80.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1050
variable_sites: 68
pi: 0.0449
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CTTTCGTCCACTCAGCCATC	ACGAATCACACTTTTACCACT	55.0	38.1	58.6	55.5	1060-1109 bp	1.0	55.6
trnS-GCU-trnG-UCC_p2	CTTTCGTCCACTCAGCCATC	GAACGAATCACACTTTTACCACT	55.0	39.1	58.6	57.3	1062-1111 bp	1.0	54.5
trnS-GCU-trnG-UCC_p3	CTTTCGTCCACTCAGCCATC	AACGAATCACACTTTTACCACT	55.0	36.4	58.6	56.2	1061-1110 bp	1.0	53.7
trnS-GCU-trnG-UCC_p4	TTTCGTCCACTCAGCCATCT	ACGAATCACACTTTTACCACT	50.0	38.1	59.0	55.5	1059-1108 bp	1.0	52.5
trnS-GCU-trnG-UCC_p5	TCCACTCAGCCATCTCTCC	ACGAATCACACTTTTACCACT	57.9	38.1	58.1	55.5	1054-1103 bp	1.0	52.0