primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	80	456	428-447	1.000000	87.7
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	80	456	475-494	1.000000	87.5
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	80	456	489-508	1.000000	87.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	80	456	488-507	1.000000	87.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	80	456	490-509	1.000000	87.3
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CTTTCGTCCACTCAGCCATC	ACGAATCACACTTTTACCACT	20	21	55.0	38.1	58.6	55.5	0.0	0.0	4.172099	9334	10383	1060-1109	1.000000	55.6
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	CTTTCGTCCACTCAGCCATC	GAACGAATCACACTTTTACCACT	20	23	55.0	39.1	58.6	57.3	0.0	0.0	4.304815	9334	10383	1062-1111	1.000000	54.5
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CTTTCGTCCACTCAGCCATC	AACGAATCACACTTTTACCACT	20	22	55.0	36.4	58.6	56.2	0.0	0.0	4.412570	9334	10383	1061-1110	1.000000	53.7
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	TTTCGTCCACTCAGCCATCT	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	59.0	55.5	0.0	0.0	4.559202	9334	10383	1059-1108	1.000000	52.5
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	TCCACTCAGCCATCTCTCC	ACGAATCACACTTTTACCACT	19	21	57.9	38.1	58.1	55.5	0.0	0.0	4.628140	9334	10383	1054-1103	1.000000	52.0
rps12-clpP_p1	rps12-clpP	1	CTGAACGAGTCGCACATACA	ATGCCTTTCTGTCAGCAACA	20	20	50.0	45.0	58.0	58.0	13.6	0.0	0.037339	75717	77272	1463-1744	1.000000	91.7
rps12-clpP_p2	rps12-clpP	2	ATCGAGCTGATTGGGTTTCC	ATGCCTTTCTGTCAGCAACA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.058650	75717	77272	1628-1909	1.000000	91.5
rps12-clpP_p3	rps12-clpP	3	CTGAACGAGTCGCACATACA	AAGCAACGGAGCCATCATAG	20	20	50.0	50.0	58.0	58.0	13.6	0.0	0.063770	75717	77272	1888-2168	1.000000	91.5
rps12-clpP_p4	rps12-clpP	4	ATCGAGCTGATTGGGTTTCC	AAGCAACGGAGCCATCATAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.085081	75717	77272	2053-2333	1.000000	91.3
rps12-clpP_p5	rps12-clpP	5	TAGTGACCGGTTCTTCGAGT	ATGCCTTTCTGTCAGCAACA	20	20	50.0	45.0	58.1	58.0	9.4	0.0	0.112936	75717	77272	1549-1830	1.000000	91.1
psbN-psbH_p1	psbN-psbH	1	GATGGTGACTAGGGTTGCTG	CACTCTCGACGGTTTGTGTA	20	20	55.0	50.0	58.0	57.6	0.0	0.0	0.440624	82027	82321	347-458	1.000000	86.8
psbN-psbH_p2	psbN-psbH	2	AGATGGTGACTAGGGTTGCT	CACTCTCGACGGTTTGTGTA	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.452497	82027	82321	348-459	1.000000	86.7
psbN-psbH_p3	psbN-psbH	3	GGTGACTAGGGTTGCTGTTT	CACTCTCGACGGTTTGTGTA	20	20	50.0	50.0	57.7	57.6	0.0	0.0	0.682729	82027	82321	344-455	1.000000	84.8
psbN-psbH_p4	psbN-psbH	4	GATGGTGACTAGGGTTGCTG	CTCTCGACGGTTTGTGTAGC	20	20	55.0	55.0	58.0	58.7	0.0	0.0	0.692018	82027	82321	345-456	1.000000	84.8
psbN-psbH_p5	psbN-psbH	5	AGATGGTGACTAGGGTTGCT	CTCTCGACGGTTTGTGTAGC	20	20	50.0	55.0	58.0	58.7	0.0	0.0	0.703891	82027	82321	346-457	1.000000	84.7
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	TTGAAGTGGGTCCATGAAGC	20	20	50.0	50.0	58.0	58.1	7.6	0.0	0.099501	94099	94508	573-582	1.000000	90.3
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GTTGGGCGCTTTAACCATTC	GGAGCGAAACAATCAGCCTA	20	20	50.0	50.0	58.0	58.0	7.6	0.0	0.033376	94099	94508	896-905	1.000000	86.9
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	GTTGGGCGCTTTAACCATTC	CCAGGAAACAACTCTCCGAG	20	20	50.0	55.0	58.0	57.9	7.6	0.0	0.095255	94099	94508	941-950	1.000000	86.1
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	AGGTCCCATTCTTCTACCCC	GGAGCGAAACAATCAGCCTA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.129476	94099	94508	1287-1296	1.000000	84.2
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	GGTCCCATTCTTCTACCCCT	GGAGCGAAACAATCAGCCTA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.129476	94099	94508	1286-1295	1.000000	84.2
rrn16-trnI-GAU_p1	rrn16-trnI-GAU	1	CAAGGTAGCCGTACTGGAAG	CGGATGGGCACATTGAACTA	20	20	55.0	50.0	57.7	58.0	7.8	1.2	0.332210	110098	110389	434-435	1.000000	84.5
rrn16-trnI-GAU_p2	rrn16-trnI-GAU	2	AGTGACTGGAGTGAAGTCGT	CGGATGGGCACATTGAACTA	20	20	50.0	50.0	58.3	58.0	0.0	1.2	0.343331	110098	110389	456-457	1.000000	84.1
rrn16-trnI-GAU_p3	rrn16-trnI-GAU	3	ACAAGGTAGCCGTACTGGAA	CGGATGGGCACATTGAACTA	20	20	50.0	50.0	58.4	58.0	7.8	1.2	0.400809	110098	110389	435-436	1.000000	84.0
rrn16-trnI-GAU_p4	rrn16-trnI-GAU	4	AAGTCGTAACAAGGTAGCCG	CGGATGGGCACATTGAACTA	20	20	50.0	50.0	57.6	58.0	0.0	1.2	0.395897	110098	110389	443-444	1.000000	83.9
rrn16-trnI-GAU_p5	rrn16-trnI-GAU	5	AACAAGGTAGCCGTACTGGA	CGGATGGGCACATTGAACTA	20	20	50.0	50.0	58.4	58.0	7.8	1.2	0.400809	110098	110389	436-437	1.000000	83.9
ycf1_p1	ycf1	1	GCATTCGTAGTTGATCCCGT	TTTCCCTCCTCCAATCCCTT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.138990	119613	119732	1075-1081	0.666667	70.1
ycf1_p2	ycf1	2	GCATTCGTAGTTGATCCCGT	TTTTCCCTCCTCCAATCCCT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.138990	119613	119732	1076-1082	0.666667	70.1
ycf1_p3	ycf1	3	GCATTCGTAGTTGATCCCGT	TGGTTCCGTGGACTCTAGTT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.069438	119613	119732	894-894	0.333333	52.8
ycf1_p4	ycf1	4	ACATCGGGGCAGAGAAGATA	TGGTTCCGTGGACTCTAGTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.093475	119613	119732	722-722	0.333333	52.6
ycf1_p5	ycf1	5	CCCCAATGGCCATACAACTT	TGGTTCCGTGGACTCTAGTT	20	20	50.0	50.0	58.1	58.0	21.1	0.0	0.140701	119613	119732	780-780	0.333333	52.2
ndhF-rpl32_p1	ndhF-rpl32	1	AGGAAAAGACCACATACGCC	AATCCCCATCCCAGAACTGA	20	20	50.0	50.0	57.9	58.0	0.0	3.2	0.123803	125855	126923	1595-1627	1.000000	88.9
ndhF-rpl32_p2	ndhF-rpl32	2	TTTGCTGCAATAGGTCGTGT	AATCCCCATCCCAGAACTGA	20	20	45.0	50.0	58.1	58.0	16.8	3.2	0.123502	125855	126923	1984-2016	0.666667	72.2
ndhF-rpl32_p3	ndhF-rpl32	3	ATCCCCCACACGATTAGTCA	AATCCCCATCCCAGAACTGA	20	20	50.0	50.0	58.1	58.0	0.0	3.2	0.140249	125855	126923	2038-2056	0.666667	69.5
ndhF-rpl32_p4	ndhF-rpl32	4	ATGAAGGGGAAATTGGGCAG	AATCCCCATCCCAGAACTGA	20	20	50.0	50.0	58.1	58.0	0.0	3.2	0.144314	125855	126923	2221-2239	0.666667	69.2
ndhF-rpl32_p5	ndhF-rpl32	5	GAAGGGGAAATTGGGCAGAT	AATCCCCATCCCAGAACTGA	20	20	50.0	50.0	57.8	58.0	0.0	3.2	0.179736	125855	126923	2219-2237	0.666667	68.9
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CAAGATCTCTCTCGACCGGA	CAGGAGCACTGCTTCCTAAG	20	20	55.0	55.0	58.3	58.0	9.5	16.9	0.351751	127089	127802	828-867	1.000000	85.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CAAGATCTCTCTCGACCGGA	AGGAGCACTGCTTCCTAAGA	20	20	55.0	50.0	58.3	58.1	9.5	16.2	0.386219	127089	127802	827-866	1.000000	85.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGCTTTTCGGCAGCATTAAA	CAGGAGCACTGCTTCCTAAG	20	20	45.0	55.0	57.6	58.0	0.0	16.9	0.383524	127089	127802	862-901	1.000000	84.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	CAAGATCTCTCTCGACCGGA	TGACCCGAATAAATCCAGCG	20	20	55.0	50.0	58.3	58.1	9.5	0.0	0.382291	127089	127802	1189-1228	1.000000	83.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGCTTTTCGGCAGCATTAAA	TGACCCGAATAAATCCAGCG	20	20	45.0	50.0	57.6	58.1	0.0	0.0	0.414065	127089	127802	1223-1262	1.000000	82.8
psaC-ndhE_p1	psaC-ndhE	1	CGGACACATTGAGTACACCC	TCGTAACAGAAAATCAACTCGT	20	22	55.0	36.4	58.3	56.1	0.0	0.0	4.197777	131090	131443	415-462	1.000000	54.9
psaC-ndhE_p2	psaC-ndhE	2	GGCTCGGACACATTGAGTAC	TCGTAACAGAAAATCAACTCGT	20	22	55.0	36.4	58.4	56.1	0.0	0.0	4.278211	131090	131443	419-466	1.000000	54.3
psaC-ndhE_p3	psaC-ndhE	3	GCTCGGACACATTGAGTACA	TCGTAACAGAAAATCAACTCGT	20	22	50.0	36.4	57.6	56.1	0.0	0.0	4.283576	131090	131443	418-465	1.000000	54.2
psaC-ndhE_p4	psaC-ndhE	4	AGGCTCGGACACATTGAGTA	TCGTAACAGAAAATCAACTCGT	20	22	50.0	36.4	58.4	56.1	0.0	0.0	4.362689	131090	131443	420-467	1.000000	53.6
psaC-ndhE_p5	psaC-ndhE	5	TCGGACACATTGAGTACACC	TCGTAACAGAAAATCAACTCGT	20	22	50.0	36.4	57.3	56.1	0.0	0.0	4.655932	131090	131443	416-463	1.000000	51.3
trnI-GAU-rrn16_p1	trnI-GAU-rrn16	1	CGGATGGGCACATTGAACTA	CAAGGTAGCCGTACTGGAAG	20	20	50.0	55.0	58.0	57.7	1.2	7.8	0.332210	150289	150580	434-435	1.000000	84.5
trnI-GAU-rrn16_p2	trnI-GAU-rrn16	2	CGGATGGGCACATTGAACTA	AGTGACTGGAGTGAAGTCGT	20	20	50.0	50.0	58.0	58.3	1.2	0.0	0.343331	150289	150580	456-457	1.000000	84.1
trnI-GAU-rrn16_p3	trnI-GAU-rrn16	3	CGGATGGGCACATTGAACTA	ACAAGGTAGCCGTACTGGAA	20	20	50.0	50.0	58.0	58.4	1.2	7.8	0.400809	150289	150580	435-436	1.000000	84.0
trnI-GAU-rrn16_p4	trnI-GAU-rrn16	4	CGGATGGGCACATTGAACTA	AAGTCGTAACAAGGTAGCCG	20	20	50.0	50.0	58.0	57.6	1.2	0.0	0.395897	150289	150580	443-444	1.000000	83.9
trnI-GAU-rrn16_p5	trnI-GAU-rrn16	5	CGGATGGGCACATTGAACTA	AACAAGGTAGCCGTACTGGA	20	20	50.0	50.0	58.0	58.4	1.2	7.8	0.400809	150289	150580	436-437	1.000000	83.9
ycf2_p1	ycf2	1	ATCGATGGGCGTGAAAAGAA	GAAATGCGTTGAGAAAGGGC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.125338	162161	162280	805-808	0.666667	70.1
ycf2_p2	ycf2	2	ATGAAATCGATGGGCGTGAA	GAAATGCGTTGAGAAAGGGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.054345	162161	162280	810-810	0.333333	52.9
ycf2_p3	ycf2	3	CGCATCCGTAGTCTCATCAG	GAAATGCGTTGAGAAAGGGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.060883	162161	162280	1483-1483	0.333333	52.8
ycf2_p4	ycf2	4	ATCGATGGGATCTGGTAGGG	GAAATGCGTTGAGAAAGGGC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.073211	162161	162280	847-847	0.333333	52.7
ycf2_p5	ycf2	5	AATCGATGGGCGTGAAAAGA	GAAATGCGTTGAGAAAGGGC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.125338	162161	162280	806-806	0.333333	52.3
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	TTGAAGTGGGTCCATGAAGC	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.1	58.0	0.0	7.6	0.099501	166170	166579	573-582	1.000000	90.4
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	GGAGCGAAACAATCAGCCTA	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.033376	166170	166579	896-905	1.000000	87.0
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	CCAGGAAACAACTCTCCGAG	GTTGGGCGCTTTAACCATTC	20	20	55.0	50.0	57.9	58.0	0.0	7.6	0.095255	166170	166579	941-950	1.000000	86.2
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	GGAGCGAAACAATCAGCCTA	AGGTCCCATTCTTCTACCCC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.129476	166170	166579	1287-1296	1.000000	84.2
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	GGAGCGAAACAATCAGCCTA	GGTCCCATTCTTCTACCCCT	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.129476	166170	166579	1286-1295	1.000000	84.2