Hotspot

matK

LSC · 2089-3609 · 1521 bp

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Pi 0

MarkerSeek score 35.90

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1521
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ATTTACAACGTTTCATAAGGAA	CCTTGTTTTGACTGTATCGCA	27.3	42.9	52.1	56.8	1621 bp	1.0	40.0
matK_p2	ATTTACAACGTTTCATAAGGAA	ACCTTGTTTTGACTGTATCGC	27.3	42.9	52.1	56.8	1622 bp	1.0	40.0
matK_p3	ATTTACAACGTTTCATAAGGAA	CCTTGTTTTGACTGTATCGCAC	27.3	45.5	52.1	58.2	1621 bp	1.0	40.0
matK_p4	ATTTACAACGTTTCATAAGGAA	CCGAGGTATCTATTCGGTTTGT	27.3	45.5	52.1	57.7	1652 bp	1.0	40.0
matK_p5	ATTTACAACGTTTCATAAGGAA	ACCTTGTTTTGACTGTATCGCA	27.3	40.9	52.1	58.5	1622 bp	1.0	40.0