Hotspot

rps8-rpl14

LSC · 80449-80646 · 198 bp

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Pi 0

MarkerSeek score 53.00

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 198
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	CTCGAACCACGCCTTTTCTA	TGCGATTGCCAGAGAATTGA	50.0	45.0	57.9	57.9	343-344 bp	1.0	78.7
rps8-rpl14_p2	CTCGAACCACGCCTTTTCTA	GTGCGATTGCCAGAGAATTG	50.0	50.0	57.9	57.8	344-345 bp	1.0	78.2
rps8-rpl14_p3	CTACTCGAACCACGCCTTTT	TGCGATTGCCAGAGAATTGA	50.0	45.0	58.2	57.9	346-347 bp	1.0	77.7
rps8-rpl14_p4	TACTCGAACCACGCCTTTTC	TGCGATTGCCAGAGAATTGA	50.0	45.0	58.2	57.9	345-346 bp	1.0	77.6
rps8-rpl14_p5	CTACTCGAACCACGCCTTTT	GTGCGATTGCCAGAGAATTG	50.0	50.0	58.2	57.8	347-348 bp	1.0	77.1