Hotspot

rps4-trnL-UAA

LSC · 45997-46936 · 940 bp

Back to Pluchea

Pi 0

MarkerSeek score 58.90

Primer available yes

Label rps4-trnL-UAA

Feature Metadata

label_name: rps4-trnL-UAA
feature_type: igs
region: LSC
length_bp: 940
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-UAA_p1	TTCCAGCTTTGGGCCTTTTA	GCGTCTACCTATTTCGCCAT	45.0	50.0	57.6	57.8	1053-1054 bp	1.0	75.8
rps4-trnL-UAA_p2	AGATCACTTCCAGCTTTGGG	GCGTCTACCTATTTCGCCAT	50.0	50.0	57.5	57.8	1060-1061 bp	1.0	74.8
rps4-trnL-UAA_p3	TCCAGCTTTGGGCCTTTTAT	GCGTCTACCTATTTCGCCAT	45.0	50.0	57.4	57.8	1052-1053 bp	1.0	73.9
rps4-trnL-UAA_p4	TTCCAGCTTTGGGCCTTTTA	AAGTCCGTAGCGTCTACCTA	45.0	50.0	57.6	57.3	1062-1063 bp	1.0	71.4
rps4-trnL-UAA_p5	TTCCAGCTTTGGGCCTTTTA	TAAGTCCGTAGCGTCTACCT	45.0	50.0	57.6	57.3	1063-1064 bp	1.0	71.4