Hotspot

trnR-UCU-DXZ06_pgt010

LSC · 29939-31147 · 1209 bp

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Pi 0
MarkerSeek score 58.80
Primer available yes
Label trnR-UCU-DXZ06_pgt010

Feature Metadata

label_name
trnR-UCU-DXZ06_pgt010
feature_type
igs
region
LSC
length_bp
1209
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-DXZ06_pgt010_p1 AGAAGACCTCTGTCCTATCCA ATTTGAACCGAGGGCTTACG 47.6 50.0 56.9 58.3 1301 bp 1.0 61.5
trnR-UCU-DXZ06_pgt010_p2 AGGTTTAGAAGACCTCTGTCCT ATTTGAACCGAGGGCTTACG 45.5 50.0 57.9 58.3 1307 bp 1.0 60.9
trnR-UCU-DXZ06_pgt010_p3 AGAAGACCTCTGTCCTATCCA GGATTTGAACCGAGGGCTTA 47.6 50.0 56.9 57.6 1303 bp 1.0 60.3
trnR-UCU-DXZ06_pgt010_p4 AGGTTTAGAAGACCTCTGTCCT GGATTTGAACCGAGGGCTTA 45.5 50.0 57.9 57.6 1309 bp 1.0 59.7
trnR-UCU-DXZ06_pgt010_p5 AGAAGACCTCTGTCCTATCCA ACGCCTTACCATGGGATTAC 47.6 50.0 56.9 57.4 1284 bp 1.0 58.6