Hotspot

trnC-GCA-psbM

LSC · 9273-10722 · 1450 bp

Back to Pluchea

Pi 0

MarkerSeek score 58.80

Primer available yes

Label trnC-GCA-psbM

Feature Metadata

label_name: trnC-GCA-psbM
feature_type: igs
region: LSC
length_bp: 1450
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-psbM_p1	GTTGTGTATCGTTTTGGCGG	TGCTACTGCACTGTTCATTT	50.0	40.0	58.0	55.6	1624-1625 bp	1.0	60.3
trnC-GCA-psbM_p2	TTTCCCCAGTTCAAATCCGG	TGCTACTGCACTGTTCATTT	50.0	40.0	58.1	55.6	1567-1568 bp	1.0	60.0
trnC-GCA-psbM_p3	GTTGTGTATCGTTTTGGCGG	TGCTACTGCACTGTTCATTTT	50.0	38.1	58.0	56.4	1624-1625 bp	1.0	58.6
trnC-GCA-psbM_p4	TTTCCCCAGTTCAAATCCGG	TGCTACTGCACTGTTCATTTT	50.0	38.1	58.1	56.4	1567-1568 bp	1.0	58.3
trnC-GCA-psbM_p5	GGGGGACTGCAAATCCTTTT	TGCTACTGCACTGTTCATTT	50.0	40.0	58.4	55.6	1585-1586 bp	1.0	57.8