Hotspot

trnK-UUU-rps16

LSC · 4385-5211 · 827 bp

Back to Pluchea

Pi 0

MarkerSeek score 58.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 827
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCGATGGTATGGACGAATCC	TTCCTTGAAAAAGGCGCTCA	55.0	45.0	57.9	58.3	1008-1009 bp	1.0	76.9
trnK-UUU-rps16_p2	CGATGGTATGGACGAATCCC	TTCCTTGAAAAAGGCGCTCA	55.0	45.0	57.9	58.3	1007-1008 bp	1.0	76.9
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	947-948 bp	1.0	76.2
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	946-947 bp	1.0	76.2
trnK-UUU-rps16_p5	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.8	58.3	1032-1033 bp	1.0	75.7