Hotspot

trnP-UGG-psaJ

LSC · 70730-71139 · 410 bp

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Pi 0.0126
MarkerSeek score 63.60
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
410
variable_sites
5
pi
0.0126
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 AAAACAAACGCGCTACCAAG GGTGCCACGGAAAGATATGT 45.0 50.0 57.9 58.0 479-480 bp 1.0 81.8
trnP-UGG-psaJ_p2 AAAACAAACGCGCTACCAAG ACGCATCCGGAAATAAACGA 45.0 45.0 57.9 58.0 553-554 bp 1.0 81.6
trnP-UGG-psaJ_p3 ATCCCTCGTTACTGCTCAGA GGTGCCACGGAAAGATATGT 50.0 50.0 57.9 58.0 552-553 bp 1.0 81.1
trnP-UGG-psaJ_p4 ATCCCTCGTTACTGCTCAGA ACGCATCCGGAAATAAACGA 50.0 45.0 57.9 58.0 626-627 bp 1.0 81.0
trnP-UGG-psaJ_p5 CTCGTTACTGCTCAGACGAG ACGCATCCGGAAATAAACGA 55.0 45.0 57.8 58.0 622-623 bp 1.0 80.9