Hotspot

ycf4-cemA

LSC · 64029-64989 · 961 bp

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Pi 0.0105
MarkerSeek score 65.40
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
961
variable_sites
10
pi
0.0105
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TTGACTCCGCGTGAAATTGA GAGATCCACCAGGGCAAAAA 45.0 50.0 58.1 58.1 1105-1107 bp 1.0 79.5
ycf4-cemA_p2 AGAATTTGACTCCGCGTGAA GAGATCCACCAGGGCAAAAA 45.0 50.0 57.8 58.1 1110-1112 bp 1.0 79.2
ycf4-cemA_p3 TATTTCTTGCGCGTACCGAT GAGATCCACCAGGGCAAAAA 45.0 50.0 57.8 58.1 1063-1065 bp 1.0 78.9
ycf4-cemA_p4 CCGCGTGAAATTGAGCAAAA GAGATCCACCAGGGCAAAAA 45.0 50.0 58.2 58.1 1099-1101 bp 1.0 78.7
ycf4-cemA_p5 GAGAATTTGACTCCGCGTGA GAGATCCACCAGGGCAAAAA 50.0 50.0 58.3 58.1 1111-1113 bp 1.0 78.2