Hotspot

trnF-GAA-ndhJ

LSC · 51792-52557 · 766 bp

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Pi 0.0131
MarkerSeek score 65.00
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
766
variable_sites
10
pi
0.0131
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA AGTTGGATAGGATGGCCCTT 55.0 50.0 58.0 58.1 908-930 bp 1.0 80.2
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA AGTTGGATAGGATGGCCCTT 50.0 50.0 58.1 58.1 872-894 bp 1.0 79.8
trnF-GAA-ndhJ_p3 CTGGTAGAGCAGAGGACTGA AGTTGGATAGGATGGCCCTT 55.0 50.0 57.9 58.1 896-918 bp 1.0 79.1
trnF-GAA-ndhJ_p4 AAGGAATGGTCGGGATAGCT AGTTGGATAGGATGGCCCTT 50.0 50.0 58.2 58.1 919-941 bp 1.0 78.6
trnF-GAA-ndhJ_p5 TGTGTAAGGAATGGTCGGGA AGTTGGATAGGATGGCCCTT 50.0 50.0 58.4 58.1 924-946 bp 1.0 77.3