Hotspot

psbZ-trnG-GCC

LSC · 38807-39669 · 863 bp

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Pi 0.013
MarkerSeek score 67.90
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
863
variable_sites
11
pi
0.013
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 GACGGTTGGTCGAGTAACAA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.9 57.8 1007-1009 bp 1.0 79.6
psbZ-trnG-GCC_p2 TTTGCTTCTCCTGACGGTTG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.4 57.8 1019-1021 bp 1.0 77.4
psbZ-trnG-GCC_p3 ATTTGCTTCTCCTGACGGTT CGTCTTCTCCTTGGCAAAGA 45.0 50.0 57.4 57.8 1020-1022 bp 1.0 76.1
psbZ-trnG-GCC_p4 ACGGTTGGTCGAGTAACAAA CGTCTTCTCCTTGGCAAAGA 45.0 50.0 57.4 57.8 1006-1008 bp 1.0 75.9
psbZ-trnG-GCC_p5 GACGGTTGGTCGAGTAACAA ACTTTAACCTGGGCGGATAG 50.0 50.0 57.9 57.0 1042-1044 bp 1.0 72.9