Hotspot

trnT-GGU-psbD

LSC · 34105-35500 · 1396 bp

Back to Plinia

Pi 0.0123
MarkerSeek score 59.70
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1396
variable_sites
17
pi
0.0123
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG ACGGTCTCTACGTAACCAGT 55.0 50.0 58.1 57.8 1523-1535 bp 1.0 79.6
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA ACGGTCTCTACGTAACCAGT 50.0 50.0 58.1 57.8 1526-1538 bp 1.0 79.4
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA ACGGTCTCTACGTAACCAGT 50.0 50.0 58.3 57.8 1500-1512 bp 1.0 77.9
trnT-GGU-psbD_p4 TGGGCCCTTTTAACTCAGTG ACGGTCTCTACGTAACCAGT 50.0 50.0 57.7 57.8 1540-1552 bp 1.0 77.8
trnT-GGU-psbD_p5 GGCCCTTTTAACTCAGTGGT ACGGTCTCTACGTAACCAGT 50.0 50.0 57.7 57.8 1538-1550 bp 1.0 77.8