Hotspot

psbM-trnD-GUC

LSC · 31446-32516 · 1071 bp

Back to Plinia

Pi 0.00843
MarkerSeek score 64.00
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1071
variable_sites
9
pi
0.00843
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAAGAGTGCAGTAGC TCAATTGGTTAGAGCACCGC 45.0 50.0 55.2 58.6 1181-1184 bp 1.0 53.9
psbM-trnD-GUC_p2 ACTAGAATGAAGAGTGCAGTAGC TCAATTGGTTAGAGCACCGC 43.5 50.0 58.0 58.6 1184-1187 bp 1.0 52.4
psbM-trnD-GUC_p3 AGGAACTAGAATGAAGAGTGCAG TCAATTGGTTAGAGCACCGC 43.5 50.0 57.9 58.6 1188-1191 bp 1.0 51.8
psbM-trnD-GUC_p4 AGGAACTAGAATGAAGAGTGCA TCAATTGGTTAGAGCACCGC 40.9 50.0 56.8 58.6 1188-1191 bp 1.0 51.1
psbM-trnD-GUC_p5 GGAACTAGAATGAAGAGTGCAGT TCAATTGGTTAGAGCACCGC 43.5 50.0 58.2 58.6 1187-1190 bp 1.0 51.1