Hotspot

petN-psbM

LSC · 30359-31340 · 982 bp

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Pi 0.0122
MarkerSeek score 52.00
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
982
variable_sites
12
pi
0.0122
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCTTGCTTGGGCTGCTTTAA ATGGAAACGTAAGAGCGGTC 45.0 50.0 58.0 58.0 1520-1524 bp 1.0 80.9
petN-psbM_p2 CTCTACGCCAGTGATTCCAC ATGGAAACGTAAGAGCGGTC 55.0 50.0 58.1 58.0 1612-1616 bp 1.0 80.3
petN-psbM_p3 CCCTTTTTGACTCTACGCCA ATGGAAACGTAAGAGCGGTC 50.0 50.0 57.8 58.0 1622-1626 bp 1.0 79.5
petN-psbM_p4 AGTAAGTCTTGCTTGGGCTG ATGGAAACGTAAGAGCGGTC 50.0 50.0 57.8 58.0 1526-1530 bp 1.0 79.4
petN-psbM_p5 TAGTGGTTGTGGAAAGTGGC ATGGAAACGTAAGAGCGGTC 50.0 50.0 58.0 58.0 2125 bp 0.5 59.8