Hotspot

atpH-atpI

LSC · 14789-15904 · 1116 bp

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Pi 0.0126
MarkerSeek score 66.30
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1116
variable_sites
14
pi
0.0126
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT TTTTTGCAACTTTAGCCGCG 45.0 45.0 58.0 58.5 1204-1214 bp 1.0 76.7
atpH-atpI_p2 ATAACGGAAGCGGCAGAAAT TAGGTGAATCCATGGAGGGT 45.0 50.0 58.0 57.4 1177-1187 bp 1.0 76.2
atpH-atpI_p3 TACCTTGACCAACTCCAGGT TTTTTGCAACTTTAGCCGCG 50.0 45.0 57.9 58.5 1260-1270 bp 1.0 76.1
atpH-atpI_p4 GTCCAATAGAAGCAAGCCCA TTTTTGCAACTTTAGCCGCG 50.0 45.0 57.9 58.5 1242-1252 bp 1.0 76.0
atpH-atpI_p5 TACCTTGACCAACTCCAGGT TAGGTGAATCCATGGAGGGT 50.0 50.0 57.9 57.4 1233-1243 bp 1.0 75.6