Hotspot

psaC-ccsA

SSC · 96553-96742 · 190 bp

Back to Plicosepalus

Pi 0.169

MarkerSeek score 76.70

Primer available yes

Label psaC-ccsA

Feature Metadata

label_name: psaC-ccsA
feature_type: igs
region: SSC
length_bp: 190
variable_sites: 31
pi: 0.169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ccsA_p1	AGAGGACTGTGTCGGTTGTA	TCCAAAAGAAACGTGGGCAT	50.0	45.0	58.0	58.3	504-800 bp	1.0	84.5
psaC-ccsA_p2	AGAGGACTGTGTCGGTTGTA	GGGAGCTGTATGGGCTAATG	50.0	55.0	58.0	57.8	552 bp	0.5	58.4
psaC-ccsA_p3	CAAATTGCTTCCGCTCCAAG	GGGAGCTGTATGGGCTAATG	50.0	55.0	58.0	57.8	575 bp	0.5	58.4
psaC-ccsA_p4	AAATTGCTTCCGCTCCAAGA	GGGAGCTGTATGGGCTAATG	45.0	55.0	58.1	57.8	574 bp	0.5	57.7
psaC-ccsA_p5	AATTGCTTCCGCTCCAAGAA	GGGAGCTGTATGGGCTAATG	45.0	55.0	58.1	57.8	573 bp	0.5	57.7