Hotspot

ycf1-psaC

SSC · 95892-96306 · 415 bp

Back to Plicosepalus

Pi 0.122

MarkerSeek score 72.60

Primer available yes

Label ycf1-psaC

Feature Metadata

label_name: ycf1-psaC
feature_type: igs
region: SSC
length_bp: 415
variable_sites: 37
pi: 0.122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-psaC_p1	TGAACCGATTCGTCTGTCTG	TACAACCGACACAGTCCTCT	50.0	50.0	57.7	58.0	901-1020 bp	1.0	86.8
ycf1-psaC_p2	ACCAAATCGATGAAAGGGGG	TACAACCGACACAGTCCTCT	50.0	50.0	57.9	58.0	1052 bp	0.5	58.8
ycf1-psaC_p3	ACCAAATCGATGAAAGGGGG	CAGTCCTCTGTTCTTGGAGC	50.0	55.0	57.9	57.9	1041 bp	0.5	58.1
ycf1-psaC_p4	ACCAAATCGATGAAAGGGGG	TTTGCTTAGCTTTGCAACCG	50.0	45.0	57.9	57.9	1013 bp	0.5	57.8
ycf1-psaC_p5	ACCAAATCGATGAAAGGGGG	GGGAGCTGTATGGGCTAATG	50.0	55.0	57.9	57.8	1584 bp	0.5	57.3