Hotspot

atpB-rbcL

LSC · 42785-43550 · 766 bp

Back to Plicosepalus

Pi 0.058

MarkerSeek score 78.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 42
pi: 0.058
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATTCGTCCGAGAGTTGCTTC	GTAAGTCCATCGGTCCACAC	50.0	55.0	58.0	58.0	1319-1349 bp	1.0	85.6
atpB-rbcL_p2	TCATAGCTACGGTCCGAACT	GTAAGTCCATCGGTCCACAC	50.0	55.0	58.0	58.0	1228-1258 bp	1.0	85.6
atpB-rbcL_p3	ACTCATAGCTACGGTCCGAA	GTAAGTCCATCGGTCCACAC	50.0	55.0	58.0	58.0	1230-1260 bp	1.0	85.5
atpB-rbcL_p4	ATTCGTCCGAGAGTTGCTTC	CGATCAAGGCTGGTAAGTCC	50.0	55.0	58.0	58.1	1331-1361 bp	1.0	85.1
atpB-rbcL_p5	ATTCGTCCGAGAGTTGCTTC	CTCTCCAGCGCATAAATGGT	50.0	50.0	58.0	58.0	1741-1771 bp	1.0	84.2