Hotspot

ycf3-trnS-GGA

LSC · 37426-38095 · 670 bp

Back to Plicosepalus

Pi 0.0753
MarkerSeek score 80.90
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
670
variable_sites
47
pi
0.0753
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 ATTTATCCGTGATCTAGGCA TTCCAATGCTACGCCTTGAA 40.0 45.0 53.6 57.8 722-729 bp 1.0 52.5
ycf3-trnS-GGA_p2 TTTATCCGTGATCTAGGCAT TTCCAATGCTACGCCTTGAA 40.0 45.0 53.6 57.8 721-728 bp 1.0 52.5
ycf3-trnS-GGA_p3 ATTTATCCGTGATCTAGGCA ACAGTGATGGGTGAAGTGTG 40.0 50.0 53.6 57.7 790-797 bp 1.0 51.5
ycf3-trnS-GGA_p4 TTTATCCGTGATCTAGGCAT ACAGTGATGGGTGAAGTGTG 40.0 50.0 53.6 57.7 789-796 bp 1.0 51.5
ycf3-trnS-GGA_p5 TTTATCCGTGATCTAGGCAT GTGATGGGTGAAGTGTGGAA 40.0 50.0 53.6 57.7 786-793 bp 1.0 51.5