Hotspot

trnY-GUA-trnE-UUC

LSC · 24541-24607 · 67 bp

Back to Plicosepalus

Pi 0.0781

MarkerSeek score 79.10

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 67
variable_sites: 5
pi: 0.0781
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	CCAGCGTAGACATATTGCCA	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.8	58.3	173-176 bp	1.0	79.6
trnY-GUA-trnE-UUC_p2	ACCAGCGTAGACATATTGCC	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.8	58.3	174-177 bp	1.0	79.6
trnY-GUA-trnE-UUC_p3	CCAGCGTAGACATATTGCCA	TCGTCTAGTGGTTCAGGACA	50.0	50.0	57.8	57.7	194-197 bp	1.0	79.1
trnY-GUA-trnE-UUC_p4	ACCAGCGTAGACATATTGCC	TCGTCTAGTGGTTCAGGACA	50.0	50.0	57.8	57.7	195-198 bp	1.0	79.1
trnY-GUA-trnE-UUC_p5	CAACGAATTTACAGTCCGCC	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.5	58.3	155-158 bp	1.0	77.8