Hotspot

atpI-rps2

LSC · 10253-10471 · 219 bp

Back to Plicosepalus

Pi 0.112
MarkerSeek score 76.90
Primer available yes
Label atpI-rps2

Feature Metadata

label_name
atpI-rps2
feature_type
igs
region
LSC
length_bp
219
variable_sites
24
pi
0.112
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-rps2_p1 TGGTGGAACATAATAGAACATCCA CGCAATTTGTGAAGGTCGTT 37.5 45.0 57.7 57.6 287-349 bp 1.0 49.5
atpI-rps2_p2 TGGTGGAACATAATAGAACATCCA TCGCAATTTGTGAAGGTCGT 37.5 45.0 57.7 58.4 288-350 bp 1.0 49.4
atpI-rps2_p3 TGGTGGAACATAATAGAACATCCA TTCGCAATTTGTGAAGGTCG 37.5 45.0 57.7 57.3 289-351 bp 1.0 47.3
atpI-rps2_p4 GGTGGAACATAATAGAACATCCA CGCAATTTGTGAAGGTCGTT 39.1 45.0 56.1 57.6 286-348 bp 1.0 47.3
atpI-rps2_p5 TGGTGGAACATAATAGAACATCC CGCAATTTGTGAAGGTCGTT 39.1 45.0 56.1 57.6 287-349 bp 1.0 47.3