Hotspot

matK-trnQ-UUG

LSC · 3540-3951 · 412 bp

Back to Plicosepalus

Pi 0.0663

MarkerSeek score 77.00

Primer available yes

Label matK-trnQ-UUG

Feature Metadata

label_name: matK-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 412
variable_sites: 26
pi: 0.0663
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnQ-UUG_p1	GCATCTTTCACCCAACAACG	GGCGTAGTCAAGTGGTAAGG	50.0	55.0	57.9	58.0	994-1008 bp	1.0	85.5
matK-trnQ-UUG_p2	GCATCTTTCACCCAACAACG	GGGCGTAGTCAAGTGGTAAG	50.0	55.0	57.9	58.0	995-1009 bp	1.0	85.5
matK-trnQ-UUG_p3	CCGAGTTCGCTCTAGGTCTA	GGCGTAGTCAAGTGGTAAGG	55.0	55.0	58.0	58.0	523 bp	0.5	59.6
matK-trnQ-UUG_p4	CCGAGTTCGCTCTAGGTCTA	GGGCGTAGTCAAGTGGTAAG	55.0	55.0	58.0	58.0	524 bp	0.5	59.6
matK-trnQ-UUG_p5	GTTTCCGAGTTCGCTCTAGG	GGCGTAGTCAAGTGGTAAGG	55.0	55.0	58.1	58.0	527 bp	0.5	59.2